Frequency of CYP3A5 alleles in pediatric patients with advanced chronic kidney disease and kidney transplant recipients at a national referral center in Lima, Peru
DOI:
https://doi.org/10.59594/iicqp.2026.v4n2.175Keywords:
Renal Insufficiency, Chronic, Kidney Transplantation, Tacrolimus, Cytochrome P-450 CYP3A, Polymorphism, Genetic, ChildAbstract
Objective: To determine the frequency of the CYP3A5*1 and CYP3A5*3 alleles in pediatric patients with advanced chronic kidney disease and kidney transplant recipients treated at a national referral center in Lima, Peru.
Methods: This was a descriptive, observational study. Patients with advanced chronic kidney disease (stages 3a, 3b, and 5) and kidney transplant recipients treated at the Instituto Nacional de Salud del Niño San Borja between February 2019 and January 2020 were included. CYP3A5*3 polymorphism genotyping was performed using PCR-RFLP. Data were analyzed using Stata version 15.
Results: A total of 50 patients were included. Twenty-seven patients (54.0%) were male, and the median age was 13.1 years (IQR: 8.6–14.9). The frequency of the CYP3A5*1 allele was 86.0%, whereas that of the CYP3A5*3 allele was 14.0%. Overall, 72.0% of the population (n = 36) had the CYP3A5*1/*1 genotype, whereas 28.0% (n = 14) had the CYP3A5*1/*3 genotype. The CYP3A5*3/*3 genotype was not observed.
Conclusions: The CYP3A5*1 allele was the most frequent in the analyzed population. The CYP3A5*3/*3 genotype, associated with the CYP3A5 non-expressor phenotype, was not observed. These results should be confirmed in future studies with larger sample sizes and complementary molecular techniques to strengthen the reliability of the findings.
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